LOCUS       sequence-50889-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Xu B, English JM, Wilsbacher JL, Stippec S, Goldsmith EJ, Cobb MH
  TITLE     WNK1, a novel mammalian serine/threonine protein kinase lacking the 
            catalytic lysine in subdomain II.
  JOURNAL   J Biol Chem. 2000 Jun 2;275(22):16795-801.
  PUBMED    10828064
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2000-06-2"; volume: "275(22)"; pages: "16795-801"
COMMENT     Sequence Label: pCMV-Wnk1
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..31
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             44..70
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttagtgaacc gtcagaatta aagaggagaa attacatatg gcttacccat acgatgttcc
       61 agattacgct gaattcttca ctccaccgga ccaaccatgt ctgacggcac cgcagagaag
      121 cagagcggca ctcctggctt cctttcgcct ccggctccag tccccaagaa tggctcaagc
      181 tccgattctt ccgtgggcga gaaactgggg gccgcggtcg ccgactccgg aattggcagg
      241 acagaggaat accggcgccg ccggcacact atggacaagg acagtcgtgg ggcggccgcc
      301 accactaccc ccacggagca ccgatttttc cgccgaagcg tcatctgcga ctccaatgcc
      361 acggcgttgg agctgccggg cctccctctc tctatccctc aacctagtgt acccgcagtg
      421 gtgcctcaga gtgctccacc cgagcctcac agagaggaaa ctttgacggc caccgttgct
      481 tcccaggtgt ctcagcaacc ctcggctgct gcctcccctg gggaacaagc cgttgtaggc
      541 tcggccaccg cgaccgtccc cagcagtacc agcaaagacc gcccggtgtc ccagcccagc
      601 ctcgtgggta gcaaagagga gccgccgccg tccagaagtg gaagtggcag cggtggtgcc
      661 agtgccaaag agccgcagga ggaaaggaac cagcaacagg atgatatcga agagctggag
      721 accaaggcag tgggaatgtc caatgacggt cgctttctca aatttgacat cgaaatcggc
      781 agaggctcct ttaagacggt ctacaaaggt ctggacacc
//
