LOCUS       sequence-50892-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Rodrigues MA, Gomes DA, Leite MF, Grant W, Zhang L, Lam W, Cheng YC,
            Bennett AM, Nathanson MH
  TITLE     Nucleoplasmic calcium is required for cell proliferation.
  JOURNAL   J Biol Chem. 2007 Jun 8. 282(23):17061-8.
  PUBMED    17420246
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Jun 8. 282(23):17061-8."
COMMENT     Sequence Label: pcDNA5-TO-PV-NES-DsRed
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..47
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     57..81
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(757..777)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 cagagctctc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct
       61 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      121 agacaccggg accgatccag cctccggact ctagcgttta aacttaagct tggtaccggg
      181 ccccccctcg aggtcgacgc attacaaaaa aaattagaag aattagaatt agatgaaatg
      241 tcgatgacag acttgctcag cgctgaggac atcaagaagg cgataggagc ctttactgct
      301 gcagactcct tcgaccacaa aaagttcttc cagatggtgg gcctgaagaa aaagagtgcg
      361 gatgatgtga agaaggtgtt ccacattctg gacaaagaca aaagtggctt cattgaggag
      421 gatgagctgg ggtccattct gaagggcttc tcctcagatg ccagagactt gtctgctaag
      481 gaaacaaaga cgctgatggc tgctggagac aaggacgggg acggcaagat tggggttgaa
      541 gaattctcca ctctggtggc cgaaagcgcg gccgcaatgg cctcctccga gaacgtcatc
      601 accgagttca tgcgcttcaa ggtgcgcatg gagggcaccg tgaacggcca cgagttcgag
      661 atcgagggcg agggcgaggg ccgcccctac gagggccaca acaccgtgaa gctgaaggtg
      721 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt ccagtacggc
      781 tccaaggtgt gcgtgaagca ccccgccgac atccccgact acaagaagct gtccttcccc
      841 gagggcttca agtggggagc gcgtgatgaa ctt
//
