LOCUS       sequence-50907-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt8A
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..60)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    86..110
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(620..631)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 tcattactaa ccggtacgcg tagaatcgag accgaggaga gggttaggga taggcttacc
       61 ttcgaaccgc gggccctcta gatcaaccac tttgtacaag aaagctgggt cggcgcgccc
      121 acccttcaga attcaggcac tgcccttacc cagggactgg gcactgcctg gggagcgtgc
      181 gcagtaatac ttgctcacca catgcctaca ctggtcacac ttgaccgtac agcaccactg
      241 gaatttgcag ttacagctgc ttatgacctc agttttcctc tcttccacct gcagcccaca
      301 ctcagtgcac aggcgcccac agctacgtcg ctcccacctg gatgtgttgt ggctgttctg
      361 taggcactca cgaccctctg tgccatagat gcccaggctg gaattgcagg tacagtaatc
      421 tggtgattcc tctaaaaaga tcagttccgc ctctgcgcta ggaaggaagg cctcagcggg
      481 cacccagtgg ccctcggcgc tgttcccagc tctcagctgc cgcttatcca tttcaatttt
      541 cagcgcctgg tcatacttgg cctttaggta gtctcccatc tcccggaact cagccagctg
      601 cagccagcat gtctgtatgc tgcagctccc agagatgcca tgacatttgc atgtcctttt
      661 catggtggct ctcactgcca gtctgccggc cctgttgttg tgaagattca tcagggctct
      721 ggcatccttc cccttctcca aactgtccac aaagagtttg gagatccttt ccccaaattc
      781 cacattgtcg ctgcagcctc cccagatcca gccatggcct cctgtttttc cattgtttga
      841 cccatcacag ccacagt
//
