LOCUS       sequence-50908-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt8B
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..61)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    87..111
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcattacta accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac
       61 cttcgaaccg cgggccctct agatcaacca ctttgtacaa gaaagctggg tcggcgcgcc
      121 cacccttcag aattcagggt tttctcccgg gtttgtgcgc agcgcccccc cgcggccgct
      181 ctgcgcggct acagaagtac ttggtgaccc tccggcggca ctgctcgcag cggactgcgc
      241 agcaccagtg gaacttgcag ttgcagctgg acacggtctc ggcccggcgc tcctccaccg
      301 ccagcccgca gtccccgcag agccggcggc agctgcggcg ttcccagcga cccagggccc
      361 gcccgcgcct taggcactct cggccttcgg tgcccagcag ccctagcgtt ttgttctcca
      421 ggcagtagtc cggggagtcc tccaggtgca ccagctcccg ggtagagatg gagcgaaagg
      481 tgtcggcgat ggcgccgcgg ccggccgcgc tgttgccagc accctgcagc aggtccacct
      541 tgagtgctgc gtggtacttc tccttcaggt gcgcgcccac ctcgcggaac tcgggcagct
      601 gcagccaaca ggtctgcgtg gtgcagctgc cagacacgcc gtggcacttg cacgtgcgtt
      661 tcatggtgcc cttcaccgcc ttgcggccag cctcgttgtt gtgcaggttc atggctgccc
      721 gtgcatcctg tcctgtttcc agggcatcga caaactgctt ggaaatcgcc tctccgaagc
      781 ccacattgtc actgcagcct ccccacagcc agccttgtcc ccccagttgc ccgttgcggg
      841 agtcatcaca gccacagtta tcaaaatctc caaggctgca gtttctagtc agggtgt
//
