LOCUS       sequence-50910-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt9A
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..61)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    87..111
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcattacta accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac
       61 cttcgaaccg cgggccctct agatcaacca ctttgtacaa gaaagctggg tcggcgcgcc
      121 cacccttcag aattcagccc ttgcaggtgt agacctcctc acgctgcgtg cactgcctgc
      181 actccacata gcagcaccaa cgcacctggc actggcaggg ccttgtcacc acccggctct
      241 gtgtgttatg gccgcggcca cagcagatgc tctcgcagtt cttctcacgg tggcacctac
      301 ggccagcggt gcccggggag aagcggccag ccaggcagaa gctaggcgag tcatccaggt
      361 gcaccagctc tggagtgcgg ggcagcgggt cgctgccacc tgcccccgag gcacggcccc
      421 gtggtgggga gatggcacct gcctcgccgg cagcttcatt ggtggtgctg cccaccttga
      481 gtgccgtctc atacttgtgc ttcagatgct tgcccacctc atggaaaggc gccaactgcc
      541 gccagcaggt ccgcaccgtg catgagcctg acacgccgtg gcacttgcag gtggtctcca
      601 ccccagcctt gatcaccttc acacccacga ggttgttgtg gaagtccaca cgggctcgca
      661 gatccttgct tgaccgtctg cccaggaact ccttgacgaa cttgctgctg tacttaaggt
      721 tgtctccgca gcccccccac tgccaggcct cacggttctc caggtcgggt gcctcatcgc
      781 aggtacagcg ctccatgcgg cccgcgctgc acgccttggc cagtgcgtgc gtcaggccag
      841 ccgaggagat ggcatagagg
//
