LOCUS       sequence-50913-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Robbins DJ, Zhen E, Owaki H, Vanderbilt CA, Ebert D, Geppert TD, 
            Cobb MH
  TITLE     Regulation and properties of extracellular signal-regulated protein 
            kinases 1 and 2 in vitro.
  JOURNAL   J Biol Chem. 1993 Mar 5;268(7):5097-106.
  PUBMED    8444886
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1993-03-5"; volume: "268(7)"; pages: "5097-106"
COMMENT     Sequence Label: NpT7-5-Erk2
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..25
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 catggcacat caccatcacc atcacgccat ggcggcggcg gcggcgggcc cggagatggt
       61 ccgcgggcag gtgttcgacg tggggccgcg ctacactaat ctctcgtaca tcggagaagg
      121 cgcctacggc atggtttgtt ctgcttatga taatctcaac aaagttcgag ttgctatcaa
      181 gaaaatcagt ccttttgagc accagaccta ctgtcagaga accctgagag agataaaaat
      241 cctactgcgc ttcagacatg agaacatcat cggcatcaat gacatcatcc gggcaccaac
      301 cattgagcag atgaaagatg tatatatagt acaggacctc atggagacag atctttacaa
      361 gctcttgaag acacagcacc tcagcaatga tcatatctgc tattttcttt atcagatcct
      421 gagaggatta aagtatatac attcagctaa tgttctgcac cgtgacctca agccttccaa
      481 cctcctgctg aacaccactt gtgatctcaa gatctgtgac tttggccttg cccgtgttgc
      541 agatccagac catgatcata cagggttctt gacagagtat gtagccacgc gttggtacag
      601 agctccagaa attatgttga attccaaggg ttataccaag tccattgata tttggtctgt
      661 gggctgcatc ctggcagaga tgctatccaa caggcctatc ttcccaggaa agcattacct
      721 tgaccagctg aatcacatcc tgggtattct tggatctcca tcacaggaag atctgaattg
      781 tataataaat ttaaaagcta gaaactattt gctttctctc ccgcacaaaa ata
//
