LOCUS       sequence-50915-e         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt10A
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..51)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    77..101
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac cttcgaaccg
       61 cgggccctct agatcaacca ctttgtacaa gaaagctggg tcggcgcgcc cacccttcag
      121 aattcacttg cagacgctga cccactcggt gatgcggcac tcttcgcaga ccacgaaaca
      181 gcaccagtgg aagcggcagt ggcagcgctc gctgcgcgtc tggcgcagga tgttgtggcc
      241 gcggccgcag cacatgctgc cgcagccatc cgagccggcg ctgctcttgt tgcacaggcg
      301 gcccacggtg cccgccgagt ccaggcgcgg ctcgcgctcg cagaagtcgg gagacttttc
      361 gaagtaaacc aggtcggcgg ggctggcccg tcggcgcggc ccgggagcgc ccggagccgg
      421 cgagggtgcc cccgctgggc ccggctccag ctggccgccg ttgcggttgt gcggccggat
      481 gagcgtggcg cggtggaagc ggctgcgcag cagcgccccc acggtgcgga actcgggcgt
      541 cacctgccag cacgtcttga gctggcagct gcctgacgtg ccgtggcact tgcacttccg
      601 ccgcatgttc tccatcactg cctgcctccc aactcggttg ttgtgaagcc tcattctcgc
      661 gtggatgtct ctgtg
//
