LOCUS       sequence-50919-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt10B
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..61)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    87..111
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcattacta accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac
       61 cttcgaaccg cgggccctct agatcaacca ctttgtacaa gaaagctggg tcggcgcgcc
      121 cacccttcag aattcactta cacacattca cccactctgt aaccttgcac tcatcacaca
      181 gcacatagca gcaccagtgg aagcggcaat ggcagcgctc aactcgtgtc tgccggagca
      241 cgttgtgccc acggccacag cacaggctgc cacagccatc caacaggcgg ctggtcttgt
      301 tgcaggcccg gccccttgtc cctggggagc ccatagtggg gtctcgctca cagaagtcag
      361 gagacttctc aaagtagacc agctctcctg agaggcgacg gggacgcaga cggggctgga
      421 aggctccaga attgcggttg tgggtatcaa tgaagatggc ccggcccagc cgctccctca
      481 acgccgcccc cactgcccgg aactctgggg ccgccctcca gcatgtcttg aactggcagc
      541 tgcctgatgt gccatgacac ttgcatttcc gcttcaggtt ttcagttacc acctggcgcc
      601 ccaccctgtt gttgtggatt cgcattcgtg cctggatgtc ccggggagct tccctggaat
      661 ccaagaaatc ccgagagaac ttctctccaa agtccatgtc atggttacag ccaccccatt
      721 cccatgtgtc ctgggggcca gggctggggc ttgagccagg gccagggctg ggcagagagt
      781 gggggaaact cttgcctcgg gacagtgcct gcagctgcag cagtttggcc ctcagccgat
      841 cctgctcacc actgcccttc cagccacagc cacagctcac cagcttgccc aggctgcag
//
