LOCUS       sequence-50923-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt2B2-V5
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..61)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    87..111
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcattacta accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac
       61 cttcgaaccg cgggccctct agatcaacca ctttgtacaa gaaagctggg tcggcgcgcc
      121 cacccttcag aatgcaggtt tggtccagcc actctgcctt cttgggggct ttgcaagtat
      181 ggacgtccac agtatttctg cattccttgc accgtacagc acagcaccag tggaatttgc
      241 actcacactg ggtaacacgg gtgactcgag ttgtgtcgta ccctcggcca cagcacatga
      301 tttcacaacc gtctgttcct tttgatgtct tgctgcagac acggcctgca gtgcctaggg
      361 aacctgcagc cttgtccaag acacagtaat ctggagagtt gtcaaagtag acaagatcag
      421 tccgggtggc acggcgatag ccttggcggg ctgcggtgaa gttggcacca tcttgggtgg
      481 ccatcacctg cacagcccca tcatagcgtc gccgcaggta atcacctgtg cggcggaaat
      541 ctgagagtgc acgccagcag gtgcgcagag tacaggaacc actcacgcca tggcacttac
      601 actccagctt cagaaaccgc cgcacagccg tgcgaccaca gcggttatta tgtaagttca
      661 tgagggcccg ggcatcctta agcctcttct ccttggcatc cacgaaggcc ttggcaaaac
      721 ggacaccgta gtggatgttg tcactgcagc caccccagtc aaagtcccca cgctggtcat
      781 ggtgtcggcc acgggtgtag gggtcacagc tgcacacact cagttcaccc tggctacagg
      841 cgcgagtaat agcgtggact acccctgctg atgagatggc atatacaaaa gctgcctct
//
