LOCUS       sequence-50925-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt4-V5
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..62)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actcattact aaccggtacg cgtagaatcg agaccgagga gagggttagg gataggctta
       61 ccttcgaacc gcgggccctc tagatcaacc actttgtaca agaaagctgg gtcggcgcgc
      121 ccacccttca gaatgcatcg gcacgtgtgc aactccacga gccgctggca ctgccggcac
      181 ttgacgaagc agcaccagtg gaatttgcag ctgcagcgtt cagccagctc cacctgcgcc
      241 gtgtggaagc cgcggccaca gcacagcagc tcacagccgt cgatggcctt ggacgtcttg
      301 ttgcatgtgc ggcccctcgt gcccagcacg ccgctgcgca tgtcctgctc acagaagtcg
      361 gggctaggct ccaagtacac caggtcctca tctgtgtgcg gcttgaactg tgcgttgcgt
      421 ggcaccagtg ccctggagga gcccacgcgg cgtggctcca cctcagtggc accatcaaac
      481 ttctccttca gtgcgtgacc cacctggcgg aagggcggca cggctcgcca gcacgtcttt
      541 acctcacagg agcctgacac cccgtggcac ttgcattcca cccgcatgtg tgtcaggatg
      601 gccttcctgc cggcctcatt gttgtggagg ttcatgaggg ctctgctgga cgaggccccc
      661 ttgcttctct cccgcacatc cacaaacgac tgtgagaaag ccacaccgta ggcgatgttg
      721 tcagagcatc ctgaccactg gaagccctgt gggctgaccc catgcactgt cctgtcacag
      781 ccgcacttct ccagctcccc ac
//
