LOCUS       sequence-50926-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Hilf RJ, Dutzler R
  TITLE     Structure of a potentially open state of a proton-activated 
            pentameric ligand-gated ion channel.
  JOURNAL   Nature. 2009 Jan 1. 457(7225):115-8.
  PUBMED    18987630
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Jan 1. 457(7225):115-8."
COMMENT     Sequence Label: pET26-MBP-GLIC
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     46..111
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     CDS             151..180
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 attccctctg aatattttgt ttaactttaa gaaggagata tacatatgaa atacctgctg
       61 ccgaccgctg ctgctggtct gctgctcctc gctgcccagc cggcgatggc aatggatatc
      121 ggaattaatt cggatccgaa ttcgagctca catcaccatc accatcacca tcaccatcac
      181 cccatgaaaa tcgaagaagg taaactggta atctggatta acggcgataa aggctataac
      241 ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt caccgttgag
      301 catccggata aactggaaga gaaattccca caggttgcgg caactggcga tggccctgac
      361 attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct gttggctgaa
      421 atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga tgccgtacgt
      481 tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct gatttataac
      541 aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct ggataaagaa
      601 ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg
      661 ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt
      721 aaagacgtgg gcgtggataa cgctggc
//
