LOCUS       sequence-50929-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Horst D, Chen J, Morikawa T, Ogino S, Kirchner T, Shivdasani RA
  TITLE     Differential WNT activity in colorectal cancer confers limited 
            tumorigenic potential and is regulated by MAPK signaling.
  JOURNAL   Cancer Res. 2012 Feb 8.
  PUBMED    22318865
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2012 Feb 8."
COMMENT     Sequence Label: TOP-GFP
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..231
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      273..282
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(345..376)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    582..699
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
       61 aagcacttgc acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt
      121 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
      181 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      241 caccccggtg aacagctcct cgcccttgct caccatggtg gcgaccggtg gatccaacag
      301 taccggaatg ccaagctttt taccaacagt accggaatgc caagctggaa ttcgagcttc
      361 cattatatac cctctagagt ctagatctcg agcccgggct agcacgcggg cgcgcccctt
      421 tgatcttacc ccctttgatc ttaccccctt tgatcttacc ccctttgatc tcgcgcccct
      481 ttgatcttac cccctttgat cttaccccct ttgatctcgc gtaagagctc ggtaccacgc
      541 gtcgtgatat caagcttctc gaggctagtc tcgtgatcga taaaattttg aatttttgta
      601 atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc
      661 cctgcactgt accccccaat cccccctttt cttttaaaag ttaaccgata ccgtcgagat
      721 ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct
      781 tcgggcctgt cgggtc
//
