LOCUS       sequence-50938-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt5A-V5
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..42)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgtagaatcg agaccgagga gagggttagg gataggctta ccttcgaacc gcgggccctc
       61 tagatcaacc actttgtaca agaaagctgg gtcggcgcgc ccacccttca gaatgcactt
      121 gcacacaaac tggtccacga tctccgtgca cttcttgcac ttgacgtagc agcaccagtg
      181 gaacttgcag tggcagcgct ccgtctgcac ggtcttgaac tggtcgtagc cacggccgca
      241 gcacatgagc tcgcagccat ccatgccctc cgacgtcttg ttgcacaggc ggccctgcgt
      301 gcccagcgag ccggtgctct cattgcgcac gcagtagtca gggctggggt cgatgtagac
      361 caggtcttgt gtggtgggcg agttgaagcg gctgttgacc tgtaccaact tgccccggct
      421 gttgagccgc atggccgccg cgctgtcgta cttctccttc agggcatcac ccaccttgcg
      481 gaagtctgcc agctgcagcc agcatgtctt caggctacat gagccggaca ccccatggca
      541 cttgcaggcc acatcagcca ggttgtacac cgtcctgcgg ccggcctcgt tgttgtgcag
      601 gttcatgagg atgcgagcac tctcgtagga gcccttggcg tggatgcgct cccgctcgcg
      661 ggcgtccacg aactccttgg caaagcggta gccatagtcg atgttgtcgc cgcagccgcc
      721 ccagagccag tcccgcggca ggtccttggg gcgcgcggcg cggctgcagc cgcaggtgga
      781 cagctcgccc tcgcggcacg cccggctcat ggcgttcacc acccctgctg cgctcac
//
