LOCUS       sequence-50939-e         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt5B-V5
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..49)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    75..99
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cggtacgcgt agaatcgaga ccgaggagag ggttagggat aggcttacct tcgaaccgcg
       61 ggccctctag atcaaccact ttgtacaaga aagctgggtc ggcgcgccca cccttcagaa
      121 tgcatttaca gatgtactgg tccacgatct ccgtgcactt cttacacctg acgaagcagc
      181 accagtggaa cttgcagtgg cagcgctcca cctgcacgct cttgaactgg ttgtagccac
      241 gcccgcagca catgagctca cagccatcca tgccctccga ggtcttgttg cagaggcggc
      301 cctgcgtgcc cagggagccc gtgctctcgt tgcgcaggca gtagtcgggg ctggggtcca
      361 catagaccag gtcctccggg gtgggctggg tgaagcggct gttgaccagc tccagccggc
      421 ccttgcgggt gacgcgcatg gcggccgcgc tgtcgtactt ctccttcagc cggtccccga
      481 ccttgcggaa ctcggccagc tgcagccagc aggtcttgag gctgcaggac cccgagacgc
      541 cgtggcattt gcaggctacg tctgccatct tatacacagc cctgcgaccg gcctcgttgt
      601 tttgca
//
