LOCUS       sequence-50942-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt10B-V5
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..54)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    80..104
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tctaccggta cgcgtagaat cgagaccgag gagagggtta gggataggct taccttcgaa
       61 ccgcgggccc tctagatcaa ccactttgta caagaaagct gggtcggcgc gcccaccctt
      121 cagaatgcac ttacacacat tcacccactc tgtaaccttg cactcatcac acagcacata
      181 gcagcaccag tggaagcggc aatggcagcg ctcaactcgt gtctgccgga gcacgttgtg
      241 cccacggcca cagcacaggc tgccacagcc atccaacagg cggctggtct tgttgcaggc
      301 ccggcccctt gtccctgggg agcccatagt ggggtctcgc tcacagaagt caggagactt
      361 ctcaaagtag accagctctc ctgagaggcg acggggacgc agacggggct ggaaggctcc
      421 agaattgcgg ttgtgggtat caatgaagat ggcccggccc agccgctccc tcaacgccgc
      481 ccccactgcc cggaactctg gggccgccct ccagcatgtc ttgaactggc agctgcctga
      541 tgtgccatga cacttgcatt tccgcttcag gttttcagtt accacctggc gccccaccct
      601 gttgttgtgg attcgcattc gtgcctggat gtcccgggga gcttccctgg aatccaagaa
      661 atcccgagag aacttctctc caaagtccat gtcatggtta cagccacccc attcccatgt
      721 gtcctggggg ccagggctgg ggcttgagcc agggccaggg ctgggcagag agtgggggaa
      781 actcttgcct cgggacagtg
//
