LOCUS       sequence-50948-e        1976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_227_Venus_wt
FEATURES             Location/Qualifiers
     source          1..1976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..65
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1086..1088,1089..1091,1092..1802)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1131..1152)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1392..1411)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1739..1760
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccatggcaca ccatcaccac catcacggga gcggggagca gaaactaata tcagaggagg
       61 atcttggctc aggaaccggt agatctgggc acatggcgga cgaggagaag ctgccgcccg
      121 gctgggagaa gcgcatgagc cgcagctcag gccgagtgta ctacttcaac cacatcacta
      181 acgccagcca gtgggagcgg cccagcggca acagcagcag tggtggcaaa aacgggcagg
      241 gggagcctgc caggaagctg cgcattctgc agtcgactag ggagcaaggg cttgtgggat
      301 cctccaccga cggcatggac gagctgtaca agggtgctag cggaggaatg gtgagcaagg
      361 gcgaggagac cacaatgggc gtaatcaagc ccgacatgaa gatcaagctg aagatggagg
      421 gcaacgtgaa tggccacgcc ttcgtgatcg agggcgaggg cgagggcaag ccctacgacg
      481 gcaccaacac catcaacctg gaggtgaagg agggagcccc cctgcccttc tcctacgaca
      541 ttctgaccac cgcgttcgcc tacggcaaca gggccttcac caagtacccc gacgacatcc
      601 ccaactactt caagcagtcc ttccccgagg gctactcttg ggagcgcacc atgaccttcg
      661 aggacaaggg catcgtgaag gtgaagtccg acatctccat ggaggaggac tccttcatct
      721 acgagataca cctcaagggc gagaacttcc cccccaacgg ccccgtgatg cagaagaaga
      781 ccaccggctg ggacgcctcc accgagagga tgtacgtgcg cgacggcgtg ctgaagggcg
      841 acgtcaagca caagctgctg ctggagggcg gcggccacca ccgcgttgac ttcaagacca
      901 tctacagggc caagaaggcg gtgaagctgc ccgactatca ctttgtggac caccgcatcg
      961 agatcctgaa ccacgacaag gactacaaca aggtgaccgt ttacgagagc gccgtggccc
     1021 gcaactccgg aacttctggt tctggtaaac ctggttctgg tgaaggttct actaaagcgg
     1081 ccgcgatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     1141 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     1201 cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc
     1261 ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
     1321 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     1381 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1441 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1501 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga
     1561 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
     1621 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1681 accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     1741 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1801 agggtaccgc tgaccaactg actgaagagt ggtcgacggt acagcggacc gcgcctgacg
     1861 taccacgtac accagtagga aaggcaaagc tttcattcca attcccgcgg gcgcgggata
     1921 tccttcaaaa aaaacttgaa gaacttgaac ttgacgaatg agatatctga ctcgag
//
