LOCUS       sequence-50956-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Martinez-Noel G, Galligan JT, Sowa ME, Arndt V, Overton TM, Harper 
            JW, Howley PM
  TITLE     Identification and proteomic analysis of distinct UBE3A/E6AP protein
            complexes.
  JOURNAL   Mol Cell Biol. 2012 May 29.
  PUBMED    22645313
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2012 May 29."
COMMENT     Sequence Label: p6896 PHAGE-P CMVT N-HA-hE6AP II wt
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(463..487)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(503..529)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      532..541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(628..652)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    662..680
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    683..701
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(703..802)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(732..752)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggaataatc ctctctttct ctacataatt caagaatttc atataccttc tcttctgtta
       61 agtaagtcac atctttaaaa tcaattctag cgcctttctt gttcattttt atctcagagc
      121 aggagttgtt gggggcacct ttcgagttct caaggtaagc tgagcttgct cctttcttgg
      181 agggatgagg atcacagagt tttgcattaa tcttataaag ctcgagggct ttaatagctg
      241 ctgcattatt atccatacga agaaaagttg gacaggaagc acaaaactca ttcgtgcagg
      301 cttcatttcc acagccctca gttaactggt ggtagtagcg ttctattaga tgctttgcag
      361 ctgctcgctt cattcggcta gcttcaatgt cgtcagactg aggttctcct gatttccaat
      421 aacactggtg cagcttctcc atggtgaagg gggcggccgc ggagcctgct tttttgtaca
      481 aacttgtttg ataacctcga gcagcgtaat ctggaacatc gtatgggtac gccatggtgg
      541 ctagcgttta aacgctagag tccggaggct ggatcggtcc cggtgtcttc tatggaggtc
      601 aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctcgtcgacg
      661 atctctatca ctgataggga gatctctatc actgataggg agagctctgc ttatatagac
      721 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
      781 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
      841 actt
//
