LOCUS       sequence-50964-e         635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Knobel PA, Kotov IN, Felley-Bosco E, Stahel RA, Marti TM
  TITLE     Inhibition of REV3 expression induces persistent DNA damage and 
            growth arrest in cancer cells.
  JOURNAL   Neoplasia. 2011 Oct;13(10):961-70.
  PUBMED    22028621
REFERENCE   2  (bases 1 to 635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neoplasia."; date: "2011-10"; volume: "13(10)"; pages: "961-70"
COMMENT     Sequence Label: pLVTHM-shREV3-4
FEATURES             Location/Qualifiers
     source          1..635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    188..221
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    305..323
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(369..388)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    552..570
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ctggactaaa agggtctgag ggatctctag ttaccaggag tcacacaaca gaacgggcac
       61 acactacttg aagcacgtca aggcaagcgt ttattgaggc ttaagcagtg ggttccctag
      121 ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag tacaagcaaa
      181 aagcagcata acttcgtata atgtatgcta tacgaagtta ttcccatgcg acggtatcga
      241 taagcttaaa aacaaagatg ctgctacatt atctcttgaa taatgtagca gcatctttgg
      301 gggatctcta tcactgatag ggaacttata agattcccaa atccaaagac atttcacgtt
      361 tatggtgatt tcccagaaca catagcgaca tgcaaatatt gcagggcgcc actcccctgt
      421 ccctcacagc catcttcctg ccagggcgca cgcgcgctgg gtgttcccgc ctagtgacac
      481 tgggcccgcg attccttgga gcgggttgat gacgtcagcg ttcgaattcc tgcagccccg
      541 actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat
      601 cactgatatg ggagtggtaa actcgacttt cactt
//
