LOCUS       sequence-50966-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Kwon GS, Hadjantonakis AK
  TITLE     Transthyretin mouse transgenes direct RFP expression or Cre-mediated
            recombination throughout the visceral endoderm.
  JOURNAL   Genesis. 2009 Jul;47(7):447-55.
  PUBMED    19415627
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis."; 
            date: "2009-07"; volume: "47(7)"; pages: "447-55"
COMMENT     Sequence Label: Ttr:mRFP1
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..28
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(join(165..179,180..188))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(192..224)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 gggccgccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg
       61 ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgcg caccttgaag
      121 cgcatgaact ccttgatgac gtcctcggag gaggccatcg gatccttatc gtcatcgtcg
      181 tacagatccc gacccatttg ctgtccacca gtcatgctag ccattggtgg cgaattcctc
      241 tccagcaccc tgggcacaga ggagacaaga gaaatgtcag tgaaatgggt cagagtaaac
      301 cggcttgttc tttccggtga tagactctgg aacaaaacca aaagtacatg ctgatcccat
      361 tatgataaca taagtgttga caattaatga gcttctttat ccagtatgac atagacagca
      421 ttgtttccaa agcaagaaca gtttctgccc ccagatgcac tgctgtgcac cagggatatg
      481 gataatgaat gttcaaaggg aaaataggcc ctctgagagc cattgctctg tttgcctctc
      541 catctgaatg gagccactga aaggtgcatg gtgcaaaaag acagaatggg ctttttcttg
      601 agatcattct aatcagctgt tgctatagta attccctctg gttgtaaaat aaccctcaca
      661 tacttcctga ctcatttaat tatactgatt aactctttta aaggactttt tcaaagactg
      721 gaatagagtg ttggccaaaa aaagacaaag aaagatcata
//
