LOCUS       sequence-50986-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pExT
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(49..82)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(234..253)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     581..602
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttgataccgc gggcccgggt tcgccaccat ggttggtatc aaagatctat aacttcgtat
       61 agcatacatt atacgaagtt atggttacaa gacaggttta aggagaccaa tagaaactgg
      121 gcatgtggag acagagaaga ctcttgggtt tctgataggc actgactctc tctgcctatt
      181 ggtctatttt cccaccctta gggtacgtcc aggagcgcac catcttcttc aaggacgacg
      241 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      301 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      361 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggcga
      421 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
      481 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      541 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      601 tgaccgccgc cgggatcact ctcggcatgg acgagcttta caaggatatc cctgaattct
      661 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
      721 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      781 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaa
//
