LOCUS       sequence-50994-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent AAV-mediated expression of tdTomato under the CAG 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  TITLE     Accessory plasmids for optogenetics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-FLEX-tdTomato
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..53
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    116..149
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(249..272)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(447..466)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     716..734
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttttgcagat tggatcccca taacttcgta taaagtatcc tatacgaagt tatatcaaaa
       61 taggaagacc aatgcttcac catcgacccg aattgccaag catcaccatc gacccataac
      121 ttcgtataat gtatgctata cgaagttata ctagggatcc ccttacttgt acagctcgtc
      181 catgccgtac aggaacaggt ggtggcggcc ctcggagcgc tcgtactgtt ccacgatggt
      241 gtagtcctcg ttgtgggagg tgatgtccag cttggtgtcc acgtagtagt agccgggcag
      301 ttgcacgggc ttcttggcca tgtagatggt cttgaactcc accaggtagt ggccgccgtc
      361 cttcagcttc agggcctggt ggatctcgcc cttcagcacg ccgtcgcggg ggtacaggcg
      421 ctcggtggag gcctcccagc ccatggtctt cttctgcatt acggggccgt cgggggggaa
      481 gttggtgccg cgcatcttca ccttgtagat cagcgtgccg tcctgcaggg aggagtcctg
      541 ggtcacggtc accagaccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc
      601 ggggaaggac agcttcttgt aatcggggat gtcggcgggg tgcttcacgt acgccttgga
      661 gccgtacatg aactgggggg acaggatgtc ccaggcgaag ggcagggggc cgcccttggt
      721 caccttcagc ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat
      781 ctcgaactcg tggccgttca tggagccctc catgcgcacc
//
