LOCUS       sequence-50996-e         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pCA-ATG-intron-tdT3Myc
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(47..80)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(330..348)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     598..617
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gataccgcgg gcccgggttc gccaccatgg ttggtatcaa agatctataa cttcgtatag
       61 catacattat acgaagttat ggttacaaga caggtttaag gagaccaata gaaactgggc
      121 atgtggagac agagaagact cttgggtttc tgataggcac tgactctctc tgcctattgg
      181 tctattttcc cacccttagg tgagtaaggg cgaggaggtc atcaaagagt tcatgcgctt
      241 caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
      301 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
      361 gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa
      421 gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga
      481 gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca
      541 ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc
      601 cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga
      661 cggcgtgctg aagggcgaga tccaccagg
//
