LOCUS       sequence-51005-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pMADM-alpha
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..55
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             62..91
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(107..140)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(188..221)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        249..353
                     /gene="bla"
                     /label=AmpR promoter
     promoter        355..712
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        355..546
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(378..398)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      563..698
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(801..820)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cattagcgag gaggacctga acagcgaaca gaaactcatt tccgaagagg atctcaactc
       61 cgagcagaag ctgatcagcg aggaggacct gagatcctga gaattcgaag ttcctatact
      121 ttctagagaa taggaacttc actagtggat cccccgggtt cgccaccatg gttggtatca
      181 aagatctata acttcgtata gcatacatta tacgaagtta ttcaggtggc acttttcggg
      241 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      301 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
      361 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
      421 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      481 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      541 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      601 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
      661 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
      721 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
      781 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
      841 ctgatgccgc cg
//
