LOCUS       sequence-51008-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Shyu YJ, Liu H, Deng X, Hu CD
  TITLE     Identification of new fluorescent protein fragments for bimolecular 
            fluorescence complementation analysis under physiological 
            conditions.
  JOURNAL   Biotechniques. 2006 Jan . 40(1):61-6.
  PUBMED    16454041
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2006 Jan . 40(1):61-6."
COMMENT     Sequence Label: pBiFC-bFosVC155
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..260)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(51..72)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(627..653)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          717..813
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
ORIGIN
        1 gccgcttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
       61 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagtttgga ctggtagctc
      121 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      181 tggtcggcga gctgcacgcc gccgtcctcg atgttgtggc ggatcttgaa gttggccttg
      241 atgccgttct tctgcttgtc gtggttcatg actttctgtt tcaggtcgtt cgggattttg
      301 cacgccggac gggtaccacc caggtcgttc gggattttgc acgccggacg gtgtgccgcc
      361 aggatgaact ccagcttttc tttttctttc agcaggttcg cgatttcggt ttgcagcgcg
      421 gatttttcgt cttccagctg gtcggtttcc gcctgcaggg tgtcggtcag ttcacgacgg
      481 cggttacggc atttcgctgc cgccattttg ttacgttcac ggcggatgcg acgtttctct
      541 tcctcttccg gggacagttg ttcaacttta ccgcgacgac cgatggactg cgcacgaccc
      601 cgaattcggg cctccatggc cataagagcg taatctggaa catcgtatgg gtacatggtg
      661 ggcccgcggg tacaattccg cagcttttag agcagaagta acacttccgt acaggcctag
      721 aagtaaaggc aacatccact gaggagcagt tctttgattt gcaccaccac cggatccggg
      781 acctgaaata aaagacaaaa agactaaact taccagttaa ctttctggtt tttcagttcc
      841 tctagtaccg gatcctctag agtccggagg ctggatcggt cccggtgtct tctatggagg
      901 tcaaaacagc gtggatg
//
