LOCUS       sequence-51009-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Shyu YJ, Liu H, Deng X, Hu CD
  TITLE     Identification of new fluorescent protein fragments for bimolecular 
            fluorescence complementation analysis under physiological 
            conditions.
  JOURNAL   Biotechniques. 2006 Jan . 40(1):61-6.
  PUBMED    16454041
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2006 Jan . 40(1):61-6."
COMMENT     Sequence Label: pBiFC-bFosVC155
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          135..231
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             295..321
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     876..897
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
       61 caccgggacc gatccagcct ccggactcta gaggatccgg tactagagga actgaaaaac
      121 cagaaagtta actggtaagt ttagtctttt tgtcttttat ttcaggtccc ggatccggtg
      181 gtggtgcaaa tcaaagaact gctcctcagt ggatgttgcc tttacttcta ggcctgtacg
      241 gaagtgttac ttctgctcta aaagctgcgg aattgtaccc gcgggcccac catgtaccca
      301 tacgatgttc cagattacgc tcttatggcc atggaggccc gaattcgggg tcgtgcgcag
      361 tccatcggtc gtcgcggtaa agttgaacaa ctgtccccgg aagaggaaga gaaacgtcgc
      421 atccgccgtg aacgtaacaa aatggcggca gcgaaatgcc gtaaccgccg tcgtgaactg
      481 accgacaccc tgcaggcgga aaccgaccag ctggaagacg aaaaatccgc gctgcaaacc
      541 gaaatcgcga acctgctgaa agaaaaagaa aagctggagt tcatcctggc ggcacaccgt
      601 ccggcgtgca aaatcccgaa cgacctgggt ggtacccgtc cggcgtgcaa aatcccgaac
      661 gacctgaaac agaaagtcat gaaccacgac aagcagaaga acggcatcaa ggccaacttc
      721 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
      781 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
      841 aaactgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      901 gccgccgg
//
