LOCUS       sequence-51021-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Acker MG, Kolitz SE, Mitchell SF, Nanda JS, Lorsch JR
  TITLE     Reconstitution of yeast translation initiation.
  JOURNAL   Methods Enzymol. 2007;430:111-45.
  PUBMED    17913637
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2007"; volume: "430"; pages: "111-45"
COMMENT     Sequence Label: pMGAHisTev5B
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcacg
       61 attacgatat cccaacgacc gaaaaccttt acttccaggg ccatatggct tctccaaaca
      121 aaaaagatct tcgttcccca atttgttgta ttttgggtca tgtcgatacc ggtaagacta
      181 aattgttaga caaaatcaga caaaccaacg ttcaaggtgg tgaagctggt ggcatcaccc
      241 aacagattgg tgccacttat ttccccatcg acgctattaa ggcaaaaact aaagttatgg
      301 ctgaatatga aaaacaaact tttgatgtcc caggtctttt ggttattgat accccaggtc
      361 acgaatcctt ctctaactta cgttcaagag gttcttcatt gtgtaacatc gcaattttgg
      421 ttattgacat tatgcatggt ttggaacaac agactattga atctatcaaa ctgttaagag
      481 atagaaaggc tccatttgtc gttgccctaa acaaaattga tagattatat gactggaaag
      541 ccattccaaa caattcattc agagactcct ttgcaaagca atcaagagct gttcaagagg
      601 aatttcaatc taggtattct aagattcaat tggaattagc tgaacaaggt ttgaattcgg
      661 aattgtattt ccaaaacaaa aatatgtcta agtatgtctc cattgtccca acatctgccg
      721 tcaccggtga gggtgttcca gatttattgt ggttgctatt agaattgacc caaaagagga
      781 tgtccaaaca attgatgtac ttgtctcacg tg
//
