LOCUS       sequence-51034-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Neerincx A, Rodriguez GM, Steimle V, Kufer TA
  TITLE     NLRC5 Controls Basal MHC Class I Gene Expression in an MHC 
            Enhanceosome-Dependent Manner.
  JOURNAL   J Immunol. 2012 May 15;188(10):4940-50. Epub 2012 Apr 6.
  PUBMED    22490867
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Immunol."; date: "2012-05-15"; volume: "188(10)"; pages: "4940-50. 
            Epub 2012 Apr 6"
COMMENT     Sequence Label: myc-NLRC5 deltaDD
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             86..115
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             119..148
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             152..181
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             185..208
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
       61 agcgtttaaa cttaagctta ccatggagca gaagctgatc tccgaggagg acctgaacga
      121 gcagaagctg atctccgagg aggacctgaa cgagcagaag ctgatctccg aggaggacct
      181 gaacctggag gtgctgttcc agggacccgg ggtacctaag gatccagtgt ggtggaattc
      241 tgcagatatc cggaagcagt gcaagaagca gcagctagag ttggccaaga agtacctgca
      301 gctcctgcgg acctctgccc agcagcgcta caggagccaa atccctgggt cagggcagcc
      361 ccacgccttc caccaggtct atgtccctcc aatcctgcgc cgggccacag catccttaga
      421 cactccggag ggggccatta tgggggacgt caaggtggaa gatggtgctg acgtgagcat
      481 ctcggacctc ttcaacacca gggttaacaa gggcccgagg gtgaccgtgc ttttggggaa
      541 ggctggcatg ggcaagacca cgctggccca ccggctctgc cagaagtggg cagagggcca
      601 tctgaactgt ttccaggccc tgttcctttt tgaattccgc cagctcaact tgatcacgag
      661 gttcctgaca ccgtccgagc tcctttttga tctgtacctg agccctgaat cggaccacga
      721 cactgtcttc cagtacctgg agaagaacgc tgaccaagtc ctgctgatct ttgatgggct
      781 agatgaggcc ctccagccta tgggtcctga t
//
