LOCUS       sequence-51038-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Rodriguez-Viciana P, Warne PH, Vanhaesebroeck B, Waterfield MD, 
            Downward J
  TITLE     Activation of phosphoinositide 3-kinase by interaction with Ras and 
            by point mutation.
  JOURNAL   EMBO J. 1996 May 15;15(10):2442-51.
  PUBMED    8665852
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "1996-05-15"; volume: "15(10)"; pages: "2442-51"
COMMENT     Sequence Label: pcDNA3-myc-p110-alpha-CAAX
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..134
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accgagctcg gatccgccac catggaacag aaactcatat
      121 cggaggagga tctacctcca agaccatcat caggtgaact gtggggcatc cacttgatgc
      181 ccccaagaat cctagtagaa tgtttactac caaatgggat gatagtgact ttagaatgcc
      241 tccgtgaggc tacgttaata acgataaagc atgaactatt taaagaagca agaaaatacc
      301 ctctccatca acttcttcaa gatgaatctt cttacatttt cgtaagtgtt acccaagaag
      361 cagaaaggga agaatttttt gatgaaacaa gacgactttg tgaccttcgg ctttttcaac
      421 cctttttaaa agtaattgaa ccagtaggca accgtgaaga aaagatcctc aatcgagaaa
      481 ttggttttgc tatcggcatg ccagtgtgtg aattcgatat ggttaaagat ccagaagtac
      541 aggacttccg aagaaatatt ctcaatgttt gtaaagaagc tgtggatctt agggatctta
      601 attcacctca tagtagagca atgtatgttt atcctccaaa tgtagaatct tcaccagaac
      661 tgccaaagca catatataat aaattggata aagggcaaat aatagtggtg atttgggtaa
      721 tagtttctcc aaataatgac aaacagaagt atactctgaa aatcaaccat gactgtgtgc
      781 cagaacaagt aattgctgaa gcaatcagga aaaaaactcg aagtatgttg ctatcatctg
      841 aacaactaaa actctgtgtt ttagaatatc agggcaagta tattttaaaa gtgtgtggat
      901 gtgatgaata cttcctagaa aaatatcctc tgagtcagta taagtatata agaagctgta
      961 taatgcttgg gaggatgccc aatttgatgc
//
