LOCUS       sequence-51059-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Rodriguez-Viciana P, Warne PH, Khwaja A, Marte BM, Pappin D, Das P, 
            Waterfield MD, Ridley A, Downward J
  TITLE     Role of phosphoinositide 3-OH kinase in cell transformation and 
            control of the actin cytoskeleton by Ras.
  JOURNAL   Cell. 1997 May 2;89(3):457-67.
  PUBMED    9150145
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1997-05-2"; volume: "89(3)"; pages: "457-67"
COMMENT     Sequence Label: pcDNA3-HA-H-Ras_wt
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             143..169
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             176..742
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=H-Ras
                     /translation="TEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKD
                     SDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIR
                     QHKLRKLNPPDESGPGCMSCKCVLS"
     promoter        complement(806..824)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(807..824)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(844..861)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ttagcgactc tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat ccactagtaa cggccgccag
      121 tgtgctggaa ttcggagcga tgtacccata cgatgttcca gattacgctc caccaacgga
      181 atataagctt gttgttgttg gcgccggcgg tgtgggcaag agtgcgctga ccatccagct
      241 gatccagaac cattttgtgg acgaatacga ccccactata gaggattcct accggaagca
      301 ggtggtcatt gatggggaga cgtgcctgtt ggacatcctg gataccgccg gccaggagga
      361 gtacagcgcc atgcgggacc agtacatgcg caccggggag ggcttcctgt gtgtgtttgc
      421 catcaacaac accaagtctt ttgaggacat ccaccagtac agggagcaga tcaaacgggt
      481 gaaggactcg gatgacgtgc ccatggtgct ggtggggaac aagtgtgacc tggctgcacg
      541 cactgtggaa tctcggcagg ctcaggacct cgcccgaagc tacggcatcc cctacatcga
      601 gacctcggcc aagacccggc agggagtgga ggatgccttc tacacgttgg tgcgtgagat
      661 ccggcagcac aagctgcgga agctgaaccc tcctgatgag agtggccccg gctgcatgag
      721 ctgcaagtgt gtgctctcct gacgcgaatt ctgcagatat ccatcacact ggcggccgct
      781 cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
      841 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgccccctc cccgtgcctt
      901 ccttgaccct gg
//
