LOCUS       sequence-51069-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Rodriguez-Viciana P, Warne PH, Khwaja A, Marte BM, Pappin D, Das P, 
            Waterfield MD, Ridley A, Downward J
  TITLE     Role of phosphoinositide 3-OH kinase in cell transformation and 
            control of the actin cytoskeleton by Ras.
  JOURNAL   Cell. 1997 May 2;89(3):457-67.
  PUBMED    9150145
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1997-05-2"; volume: "89(3)"; pages: "457-67"
COMMENT     Sequence Label: pSG5-HA-H-Ras_wt
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..62
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             69..635
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=H-Ras
                     /translation="TEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKD
                     SDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIR
                     QHKLRKLNPPDESGPGCMSCKCVLS"
     polyA_signal    668..789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(705..724)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     759..778
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(823..840)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtatcgactc actatagggc gaattcggag cgatgtaccc atacgatgtt ccagattacg
       61 ctccaccaac ggaatataag cttgttgttg ttggcgccgg cggtgtgggc aagagtgcgc
      121 tgaccatcca gctgatccag aaccattttg tggacgaata cgaccccact atagaggatt
      181 cctaccggaa gcaggtggtc attgatgggg agacgtgcct gttggacatc ctggataccg
      241 ccggccagga ggagtacagc gccatgcggg accagtacat gcgcaccggg gagggcttcc
      301 tgtgtgtgtt tgccatcaac aacaccaagt cttttgagga catccaccag tacagggagc
      361 agatcaaacg ggtgaaggac tcggatgacg tgcccatggt gctggtgggg aacaagtgtg
      421 acctggctgc acgcactgtg gaatctcggc aggctcagga cctcgcccga agctacggca
      481 tcccctacat cgagacctcg gccaagaccc ggcagggagt ggaggatgcc ttctacacgt
      541 tggtgcgtga gatccggcag cacaagctgc ggaagctgaa ccctcctgat gagagtggcc
      601 ccggctgcat gagctgcaag tgtgtgctct cctgacgcga attcggatcc agatcttatt
      661 aaagcagaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
      721 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
      781 tgtatcttat catgtctggt cgactctaga ctcttccgct tcctcgctca ctgactcgct
      841 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
//
