LOCUS       sequence-51088-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  TITLE     Downward Lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEXV3-Myc-H-RAS_N17
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(32..52)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        37..366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      217..352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          405..501
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             555..581
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             590..619
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ttgacagctt atcatcgata agcttggctg tggaatgtgt gtcagttagg gtgtggaaag
       61 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      301 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      361 tgcaaaaagc tcctcgagga actgaaaaac cagaaagtta actggtaagt ttagtctttt
      421 tgtcttttat ttcaggtccc ggatccggtg gtggtgcaaa tcaaagaact gctcctcagt
      481 ggatgttgcc tttacttcta ggcctgtacg gaagtgttac ttctgctcta aaagctgcgg
      541 aattcggagc gatgtaccca tacgatgttc cagattacgc tccaccatgg aacagaaact
      601 catttccgaa gaggatctga agcttgttgt tgttggcgcc ggcggtgtgg gcaagaatgc
      661 gctgaccatc cagctgatcc agaaccattt tgtggacgaa tacgacccca ctatagagga
      721 ttcctaccgg aagcaggtgg tcattgatgg ggagacgtgc ctgttggaca tcctggatac
      781 cgccggccag gaggagtaca gcgccatgcg ggaccagtac atgcgcaccg gggagggctt
      841 cctgtgtgtg tttgccatca acaa
//
