LOCUS       sequence-51089-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Watton SJ, Downward J
  TITLE     Akt/PKB localisation and 3' phosphoinositide generation at sites of 
            epithelial cell-matrix and cell-cell interaction.
  JOURNAL   Curr Biol. 1999 Apr 22;9(8):433-6.
  PUBMED    10226029
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "1999-04-22"; volume: "9(8)"; pages: "433-6"
COMMENT     Sequence Label: pcDNA3-mAkt-ER
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      121..130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             127..168
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
ORIGIN
        1 tagcagagct ctctggctac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccggtaccc cggggaattc
      121 gccgccatgg ggagtagcaa gagcaagcct aaggacccca gccagcgcag cgaggctgaa
      181 gagatggagg tgtccctggc caagcccaag caccgcgtga ccatgaacga gtttgagtac
      241 ctgaagctgc tgggcaaggg cactttcggc aaggtgatcc tggtgaagga gaaggccaca
      301 ggccgctact acgccatgaa gatcctcaag aaggaagtca tcgtggccaa ggacgaggtg
      361 gcccacacac tcaccgagaa ccgcgtcctg cagaactcca ggcacccctt cctcacagcc
      421 ctgaagtact ctttccagac ccacgaccgc ctctgctttg tcatggagta cgccaacggg
      481 ggcgagctgt tcttccacct gtcccgggaa cgtgtgttct ccgaggaccg ggcccgcttc
      541 tatggcgctg agattgtgtc agccctggac tacctgcact cggagaagaa cgtggtgtac
      601 cgggacctca agctggagaa cctcatgctg gacaaggacg ggcacattaa gatcacagac
      661 ttcgggctgt gcaaggaggg gatcaaggac ggtgccacca tgaagacctt ttgcggcaca
      721 cctgagtacc tggcccccga ggtgctggag gacaatgact acggccgtgc agtggactgg
      781 tgggggctgg gcgtggtcat gtacgagatg atgtgcggtc gcctgccctt ctacaaccag
      841 gaccatgaga agctttttga gctcatcctc atggaggaga tccgcttccc gcgcacgctt
      901 ggtcccgagg ccaagtcctt gctttcaggg ctgctca
//
