LOCUS       sequence-51096-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Werner TP, Amrhein N, Freimoser FM
  TITLE     Specific localization of inorganic polyphosphate (poly P) in fungal 
            cell walls by selective extraction and immunohistochemistry.
  JOURNAL   Fungal Genet Biol. 2007 Sep;44(9):845-52. Epub 2007 Jan 20.
  PUBMED    17320430
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2007-09"; volume: "44(9)"; pages: "845-52. Epub
            2007 Jan 20"
COMMENT     Sequence Label: pETM41-EcLonA
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(607..627)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(682..705)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 tctttcggct tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattcgga tccggtacct caggccggat cgccacgcat
      121 gtcagaagac attttgtcga tctcatcgag caggaacagc gggtttttca cgcccacttt
      181 cgccattttc tggatcagtt tacccggcat agaaccgatg taagtacggc ggtgaccacg
      241 gatttccgct tcatcacgca cgccgcccag cgccatacgg acatatttac gcccggtggc
      301 tttggcaatg gactgaccaa gagaggtttt acctaccccc ggcggcccta ccaggcagag
      361 gatcggtccc ttgattttgt tgacacggct ttgaaccgca agatactcaa ggattcgatc
      421 tttcacgcgc tccagaccat aatggtcggt atcaaggatt tcctgcgcct gacgcaggtc
      481 ttttttgacc ttgctacgcg cattccacgg cacctgtacc atccagtcga tataaccacg
      541 cactacggtc gcttctgccg acatcggaga catcattttc agcttctgca actctgcttc
      601 catggcgccc tgaaaataaa gattctcgct catggggttg ttgttattgt tattgttgtt
      661 gttgttcgag ctcgaattag tctgcgcgtc tttcagggct tcatcgacag tctgacgacc
      721 gctggcggcg ttgatcaccg cagtacgcac ggcataccag aaagcggaca tctgcgggat
      781 gttcggcatg atttcacctt tctgggcgtt ttccatagtg gcggcaatac gtggatcttt
      841 cgccaactct tcctcgtaag acttcagcgc tacggcaccc agcggtttgt ctttattaac
      901 cgcttccaga cct
//
