LOCUS       sequence-51112-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Bahler J, Wu JQ, Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, 
            Wach A, Philippsen P, Pringle JR
  TITLE     Heterologous modules for efficient and versatile PCR-based gene 
            targeting in Schizosaccharomyces pombe.
  JOURNAL   Yeast. 1998 Jul;14(10):943-51.
  PUBMED    9717240
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "943-51"
COMMENT     Sequence Label: pFA6a-kanMX6-P3nmt1-GST
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..696)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(63..85)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 ggtcgacgga tctggcgcgc ctcaacgcgg aaccagatcc gattttggag gatggtcgcc
       61 accaccaaac gtggcttgcc agccctgcaa aggccatgct atatacttgc tggatttcaa
      121 gtacttatca atttgtggga tagcttcaat acgtttttta aaacaaacta attttgggaa
      181 cgcatccagg cacattgggt ccatgtataa aacaacatca agagcgtcat acaacatgaa
      241 gtcaggatgg gttacatgat caccatttaa atatgtttta tgacataaac gatcttcgaa
      301 cattttcagc atttcaggta gcttgctaag aaaatcaact ttgagagttt caaagtcttt
      361 actatatgca attctcgaaa caccgtatct aatatccaaa accgctcctt caagcattga
      421 aatctctgca cgctcttttg gacaaccacc caacatgttg tgcttgtcag ctatataacg
      481 tatgatggcc atagactgtg ttaatttaac atcaccatca atataataag gaagattggg
      541 aaactccaaa cccaattcaa actttttgtt tcgccattta tcaccttcat cgcgctcata
      601 caaatgctct tcatattttt cttcaagata ttccaaaaga agtcgagtgg gttgcacaag
      661 gcccttaatt ttccaataac ctagtatagg ggacatatta attaaagaca tgatttaaca
      721 aagcgactat aagtcagaaa gtgagaatga gattgaaata attaattcaa cttattcaat
      781 tgatgatatg ccaggattcc tcttccttta tatatcttca atgtttcgat taacaggtcc
      841 attatccggt tttt
//
