LOCUS       sequence-51115-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Watt S, Mata J, Lopez-Maury L, Marguerat S, Burns G, Bahler J
  TITLE     urg1: a uracil-regulatable promoter system for fission yeast with 
            short induction and repression times.
  JOURNAL   PLoS One. 2008 Jan 16;3(1):e1428.
  PUBMED    18197241
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2008-01-16"; volume: "3(1)"; pages: "e1428"
COMMENT     Sequence Label: pFA6a-kanMX6-Purg1-GST
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..684)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(51..73)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 tggcgcgcct caacgcggaa ccagatccga ttttggagga tggtcgccac caccaaacgt
       61 ggcttgccag ccctgcaaag gccatgctat atacttgctg gatttcaagt acttatcaat
      121 ttgtgggata gcttcaatac gttttttaaa acaaactaat tttgggaacg catccaggca
      181 cattgggtcc atgtataaaa caacatcaag agcgtcatac aacatgaagt caggatgggt
      241 tacatgatca ccatttaaat atgttttatg acataaacga tcttcgaaca ttttcagcat
      301 ttcaggtagc ttgctaagaa aatcaacttt gagagtttca aagtctttac tatatgcaat
      361 tctcgaaaca ccgtatctaa tatccaaaac cgctccttca agcattgaaa tctctgcacg
      421 ctcttttgga caaccaccca acatgttgtg cttgtcagct atataacgta tgatggccat
      481 agactgtgtt aatttaacat caccatcaat ataataagga agattgggaa actccaaacc
      541 caattcaaac tttttgtttc gccatttatc accttcatcg cgctcataca aatgctcttc
      601 atatttttct tcaagatatt ccaaaagaag tcgagtgggt tgcacaaggc ccttaatttt
      661 ccaataacct agtatagggg acatattaat taacctttgt tcagtggcaa gcatattgaa
      721 ttagttctaa tttagtaatt gaagaaaacg gataagtaaa acaacaagaa aaatgacaaa
      781 cgaatgttca atgggtagaa agaaattcgt ttcacaaaat tagcctttaa acaaagtgaa
      841 ttgagtaagg cagctaaagt aatgaatgaa ctt
//
