LOCUS       sequence-51117-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Watt S, Mata J, Lopez-Maury L, Marguerat S, Burns G, Bahler J
  TITLE     urg1: a uracil-regulatable promoter system for fission yeast with 
            short induction and repression times.
  JOURNAL   PLoS One. 2008 Jan 16;3(1):e1428.
  PUBMED    18197241
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2008-01-16"; volume: "3(1)"; pages: "e1428"
COMMENT     Sequence Label: pFA6a-kanMX6-Purg1-GFP
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(37..224)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     145..169
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(288..308)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cggatctata ttaccctgtt atccctagcg gatctgccgg tagaggtgtg gtcaataaga
       61 gcgacctcat actatacctg agaaagcaac ctgacctaca ggaaagagtt actcaagaat
      121 aagaattttc gttttaaaac ctaagagtca ctttaaaatt tgtatacact tatttttttt
      181 ataacttatt taataataaa aatcataaat cataagaaat tcgcttattt agaagtggcg
      241 cgccctattt gtatagttca tccatgccat gtgtaatccc agcagctgtt acaaactcaa
      301 gaaggaccat gtggtctctc ttttcgttgg gatctttcga aagggcagat tgtgtggaca
      361 ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat
      421 ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct aattttgaag ttaactttga
      481 ttccattctt ttgtttgtct gccatgatgt atacattgtg tgagttatag ttgtattcca
      541 atttgtgtcc aagaatgttt ccatcttctt taaaatcaat accttttaac tcgattctat
      601 taacaagggt atcaccttca aacttgactt cagcacgtgt cttgtagttc ccgtcatctt
      661 tgaaaaatat agttctttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat
      721 gccgtttcat atgatctggg tatcttgaaa agcattgaac accataagtg aaagtagtga
      781 caagtgttgg ccatggaaca ggtagttttc cagtagtgca aataaattta agggtaagtt
      841 ttccgtatgt tgcatcacct tcaccctctc cactgacaga aaatttgtgc c
//
