LOCUS       sequence-51127-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  TITLE     Li-Huei Tsai's Plasmids.
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV-P35
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(274..293)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    339..394
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(393..412)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(752..768)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(752..768)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(765..787)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    776..792
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggtggagagc tgcaaggagg ccttttggga ccgttgcctc tctgtcatca acctcatgag
       61 ctcaaagatg ctgcagataa atgccgaccc acactacttc acacaggtct tctccgacct
      121 gaagaacgag agcggccagg aggacaagaa gcggctcctc ctaggcctgg atcggtgagg
      181 atccgtcgag gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
      241 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
      301 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
      361 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
      421 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
      481 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
      541 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
      601 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
      661 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
      721 cctcgacgga tcggccgcaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
      781 atccgctcac aattcc
//
