LOCUS       sequence-51146-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pCA-T-intron(Neo)-G(LNL)
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    127..160
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      177..186
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(238..257)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(319..341)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacntatt
       61 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
      121 acctgaataa cttcgtataa tgtatgctat acgaagttat agatctttga taccaaccat
      181 ggtggcgaac ccgggcccgc ggtatcaaat caattctttg ccaaaatgat gagacagcac
      241 aataaccagc acgttgccca ggagctgtag gaaaaagaag aaggcatgaa catggttagc
      301 agaggctcta gagccgccgg tcacacgcca gaagccgaac cccgccctgc cccgtccccc
      361 ccgaaggcag ccgtcccccc gcggacagcc ccgaggctgg agagggagaa ggggacggcg
      421 gcgcggcgac gcacgaaggc cctccccgcc catttccttc ctgccggcgc cgcaccgctt
      481 cgccccgcgc ccgctagagg gggtgcggcg gcgcctccca gatttcggct ccgcacagat
      541 ttgggacaaa ggaagtccct gcgccctctc gcacgattac cataaaaggc aatggctgcg
      601 gctcgccgcg cctcgacagc cgccggcgct ccgggggccg ccgcgcccct cccccgagcc
      661 ctccccgg
//
