LOCUS       sequence-51158-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Teng L, Mundell NA, Frist AY, Wang Q, Labosky PA
  TITLE     Requirement for Foxd3 in the maintenance of neural crest 
            progenitors.
  JOURNAL   Development. 2008 May;135(9):1615-24. Epub 2008 Mar 26.
  PUBMED    18367558
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2008-05"; volume: "135(9)"; pages: "1615-24. 
            Epub 2008 Mar 26"
COMMENT     Sequence Label: pFoxd3floxtv
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..549)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(98..117)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     491..514
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(564..597)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    599..632
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgcgtgcaat ccatcttgtt caatggccga tcccatattg gctgcaggtc gaaaggcccg
       61 gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg
      121 ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc
      181 cggacccacc ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc
      241 cgctgcccca aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac
      301 tagtgagacg tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg
      361 ggaacttcct gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag
      421 ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc
      481 gccaagtgcc cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct
      541 cccctacccg gtagaattag cttgaagttc ctatactttc tagagaatag gaacttcgat
      601 aacttcgtat aatgtatgct atacgaagtt atgcggccgc ggccagtgag gcctgttcgg
      661 aagacagtcg tcggcctgtg aggcctgacc agaagacagt cgacggtat
//
