LOCUS       sequence-51161-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Thoreen CC, Chantranupong L, Keys HR, Wang T, Gray NS, Sabatini DM
  TITLE     A unifying model for mTORC1-mediated regulation of mRNA translation.
  JOURNAL   Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083.
  PUBMED    22552098
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-05-2- 2"; volume: "485"; issue: "7396"; pages:
            "109-13"
COMMENT     Sequence Label: pLJM60-eIF4E
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        220..238
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     220..237
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     251..270
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             329..352
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
       61 ccgatccagc ctccgcggcc gggaacggtg cattggaacg cggattcccc gtgccaagag
      121 tgacgtaagt accgcctata gagtctatag gcccaccccc ttggcttcgt tagaacgcgg
      181 ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac actatagaat
      241 aacatccact ttgcctttct ctccacaggt gtccactccc aggtccaact gcacctcggt
      301 tctatcgatt gaattcacca tggaaatgga ctacaaggat gacgatgaca agggtggtgg
      361 tggtggtgcg tcgacgatgg cgactgtgga accggaaacc acccctacca ctaatccccc
      421 acctgcagaa gaggaaaaaa cagagtctaa tcaggaggtt gctaacccag agcactatat
      481 taaacaccct ctacagaaca ggtgggcact ctggtttttt aaaaatgata aaagcaaaac
      541 ttggcaagca aaccttcgat tgatctctaa gtttgatact gttgaagact tttgggctct
      601 atacaaccat atccagttgt ctagtaattt aatgcctggc tgtgactact cactttttaa
      661 ggacgggatt gagcctatgt gggaagatga gaaaaacaaa cgaggaggac ggtggctgat
      721 cacactgaac aagcagcaga gacggagtga cctcgatcgc ttctggctag agacactgct
      781 gtgcct
//
