LOCUS       sequence-51167-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Kast P
  TITLE     pKSS--a second-generation general purpose cloning vector for 
            efficient positive selection of recombinant clones.
  JOURNAL   Gene. 1994 Jan 28;138(1-2):109-14.
  PUBMED    8125286
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1994-01-28"; volume: "138(1-2)"; pages: "109-14"
COMMENT     Sequence Label: pKSS
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     47..63
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     48..64
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga ggtcgacggt
       61 atcgataagc ttgatatcga attcccccgg gaccaaaatg gcaagtaaaa tagcctgatg
      121 ggataggctc taagtccaac gaaccagtgt caccactgac acaatgagga aaaccatgtc
      181 acatctcgca gaactggttg ccagtgcgaa ggcggccatt agccaggcgt cagatgttgc
      241 cgcgttagat aatgtgcgcg tcgaatattt gggtaaaaaa gggcacttaa cccttcagat
      301 gacgaccctg cgtgagctgc cgccagaaga gcgtccggca gctggtgcgg ttatcaacga
      361 agcgaaagag caggttcagc aggcgctgaa tgcgcgtaaa gcggaactgg aaagcgctgc
      421 actgaatgcg cgtctggcgg cggaaacgat tgatgtctct ctgccaggtc gtcgcattga
      481 aaacggcggt ctgcatccgg ttacccgtac catcgaccgt atcgaaagtt tcttcggtga
      541 gcttggcttt accgtggcaa ccgggccgga aatcgaagac gattatcata acttcgatgc
      601 tctgaacatt cctggtcacc acccggcgcg cgctgaccac gacactttct ggtttgacac
      661 tacccgcctg ctgcgtaccc agacctctgg cgtacagatc cgcaccatga aagcccagca
      721 gccaccgatt cgtatcatcg cgcctggccg tgtttatcgt aacgactacg accagactca
      781 cacgccgatg ttccatcaga tggaaggtct gattgt
//
