LOCUS       sequence-51170-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   MacBeath G, Kast P
  TITLE     UGA read-through artifacts--when popular gene expression systems 
            need a pATCH.
  JOURNAL   Biotechniques. 1998 May;24(5):789-94.
  PUBMED    9591128
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "1998-05"; volume: "24(5)"; pages: "789-94"
COMMENT     Sequence Label: pATCH1
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..23
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(92..110)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      106..153
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      190..645
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(277..296)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     487..508
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        671..775
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgagcacca ccaccaccac cactaataat gactagtcag ctgatccggc tgctaacaaa
       61 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
      121 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg
      181 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      241 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      301 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
      361 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
      421 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
      481 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
      541 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
      601 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
      661 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      721 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      781 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      841 tgctcaccca gaaacgctgg tgaaagtaaa agatgctga
//
