LOCUS       sequence-51171-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Thoreen CC, Chantranupong L, Keys HR, Wang T, Gray NS, Sabatini DM
  TITLE     A unifying model for mTORC1-mediated regulation of mRNA translation.
  JOURNAL   Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083.
  PUBMED    22552098
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-05-2- 2"; volume: "485"; issue: "7396"; pages:
            "109-13"
COMMENT     Sequence Label: pLJM60-Auf1
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        218..236
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     218..235
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     249..268
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             327..350
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      460..469
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
       61 gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt gccaagagtg
      121 acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta gaacgcggct
      181 acaattaata cataacctta tgtatcatac acatacgatt taggtgacac tatagaataa
      241 catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc acctcggttc
      301 tatcgattga attcaccatg gaaatggact acaaggatga cgatgacaag ggtggtggtg
      361 gtggtgcgtc gacgatgtcg gaggagcagt tcggcgggga cggggcggcg gcagcggcaa
      421 cggcggcggt aggcggctcg gcgggcgagc aggagggagc catggtggcg gcgacacagg
      481 gggcagcggc ggcggcggga agcggagccg ggaccggggg cggaaccgcg tctggaggca
      541 ccgaaggggg cagcgccgag tcggaggggg cgaagattga cgccagtaag aacgaggagg
      601 atgaagggaa aatgtttata ggaggcctta gctgggacac tacaaagaaa gatctgaagg
      661 actacttttc caaatttggt gaagttgtag actgcactct gaagttagat cctatcacag
      721 ggcgatcaag gggttttggc tttgtgctat ttaaagaatc ggagagtgta gataaggtca
      781 t
//
