LOCUS       sequence-51176-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RECQL
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..42)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(43..60)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(61..81)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tcgaatttcg gatcctcact tatcgtcgtc atccttgtaa tcatggtggt gatgatggtg
       61 cgattggaag tagaggttct ctgcctccat ctttttatca ctttgttcag aatgacaagt
      121 ttgagacgat tcagccctga aagagttctg cgtggacttt gtcacttgca tagtaatagc
      181 atgtgcctca ttgttcagaa ggttagcttt aggtcctatt ttcaaatacg aaatggtagc
      241 ataagctgta aaactgtagt cttctttaag atactgctgt attagaaagt gtgcaataat
      301 cttctccaga tcttcacgag gaagtgtggg agccacaaca cctgctactc tcagttttgc
      361 tgcacccttt cccatccaag aatcaatcag tttcaatgga gtgagttttt cattcagttc
      421 ctctgcctgc ttcaggatct tgattagatc tctgcagtac tctgttatgt tctttctttc
      481 aaatgcactg tctttacagc agttatcgca cattttgtta catgcttctg agttccatac
      541 ttcatcaaaa tgttgagcca tcaacacacg acgacatttg cttatgtttt gacagtatga
      601 taccatctca taaagcttct gctgtcccac attttccatc accaccattg aacttattct
      661 gaatatatct ccaaagccgt agtacaaaat acagtctgct ttcatgtcat ctcgacctgc
      721 acgtccactc tcttggtaat aattttccat ggatttactc attgaatgat ggataacaaa
      781 cctcacatct ggcttatcaa ttcccatacc aaatgcaaca gttgccacta ctacctgaat
      841 ttcattggct gaccattttc tatg
//
