LOCUS       sequence-51184-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Mitchell SF, Walker SE, Algire MA, Park EH, Hinnebusch AG, Lorsch JR
  TITLE     The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to 
            stimulate canonical translation initiation and to block an 
            alternative pathway.
  JOURNAL   Mol Cell. 2010 Sep 24;39(6):950-62.
  PUBMED    20864040
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2010-09-24"; volume: "39(6)"; pages: "950-62"
COMMENT     Sequence Label: pET-DUET-GST-eIF4G1-His6, eIF4E
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             199..216
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     291..310
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        291..309
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    310..334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgtacgaaca actccagtaa tagacactct ttcagaaggg acgctcctcc tgcatcaaaa
       61 gatagcttta tcacaaccag aacatattct caaagaaata gccaaagggc tcctcctcca
      121 aaggaagaac cagctgcacc aacttctacc gcaacaaata tgttcagtgc attaatggga
      181 gaaagtgatg acgaagagca tcatcatcat catcattgaa agcttgcggc cgcataatgc
      241 ttaagtcgaa cagaaagtaa tcgtattgta cacggccgca taatcgaaat taatacgact
      301 cactataggg gaattgtgag cggataacaa ttccccatct tagtatatta gttaagtata
      361 agaaggagat atacatatgt ccgttgaaga agttagcaag aagtttgaag aaaacgtttc
      421 agtcgatgat accacagcta ctccaaagac tgttttaagt gacagtgctc acttcgatgt
      481 caagcaccca ttgaacacca aatggacttt atggtacaca aagccagccg tcgataaatc
      541 tgagtcgtgg tctgatctat tacgtcccgt cacttcattc caaactgttg aagaattttg
      601 ggctatcatt caaaatattc ctgagccaca cgaactacca ttgaaatcag attaccacgt
      661 cttccgtaat gacgttagan ctgaatggga agatgaagcc aatgctaaag gtggtaaatg
      721 gtctttccaa cttagaggaa aaggtgctga tattgatgaa ttatggctaa
//
