LOCUS       sequence-51188-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Thoreen CC, Chantranupong L, Keys HR, Wang T, Gray NS, Sabatini DM
  TITLE     A unifying model for mTORC1-mediated regulation of mRNA translation.
  JOURNAL   Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083.
  PUBMED    22552098
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-05-2- 2"; volume: "485"; issue: "7396"; pages:
            "109-13"
COMMENT     Sequence Label: pLJM60-Cnbp
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        237..255
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     237..254
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     268..287
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             346..369
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cagagctcgt ttagtgaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
       61 catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat tggaacgcgg
      121 attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc cacccccttg
      181 gcttcgttag aacgcggcta caattaatac ataaccttat gtatcataca catacgattt
      241 aggtgacact atagaataac atccactttg cctttctctc cacaggtgtc cactcccagg
      301 tccaactgca cctcggttct atcgattgaa ttcaccatgg aaatggacta caaggatgac
      361 gatgacaagg gtggtggtgg tggtgcgtcg acgatgagca gcaacgaatg cttcaagtgt
      421 ggacgatctg gccactgggc cagggagtgc cctactggtg gaggtcgggg tcgtggaatg
      481 agaagccgcg gcagaggtgg ttttacctcg gatagagggt tccagtttgt ttcctcgtct
      541 ctccctgaca tctgctaccg ctgtggtgag tctggtcatc ttgccaagga ttgtgatctg
      601 caggaggatg cctgctataa ctgcggtaga ggtggccaca ttgccaagga ctgcaaggag
      661 cccaagagag agcgagagca atgctgctac aattgtggca agccaggcca tctggctcgt
      721 gactgtgacc acgcggatga gcagaagtgc tattcttgtg gtgaatttgg acatattca
//
