LOCUS       sequence-51207-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DMPK
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..107
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     RBS             263..271
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctctgaataa ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc
       61 attcttctgg tgtagatctg ggtaccgaga acctgtactt ccaatccatg cagcagctgg
      121 tgttggaccc gggcttcctg gggctggagc ccctgctcga ccttctcctg ggcgtccacc
      181 aggagctggg cgcctccgaa ctggcccagg acaagtacgt ggccgacttc ttgcagtggg
      241 cggagcccat cgtggtgagg cttaaggagg tccgactgca gagggacgac ttcgagattc
      301 tgaaggtgat cggacgcggg gcgttcagcg aggtagcggt agtgaagatg aagcagacgg
      361 gccaggtgta tgccatgaag atcatgaaca agtgggacat gctgaagagg ggcgaggtgt
      421 cgtgcttccg tgaggagagg gacgtgttgg tgaatgggga ccggcggtgg atcacgcagc
      481 tgcacttcgc cttccaggat gagaactacc tgtacctggt catggagtat tacgtgggcg
      541 gggacctgct gacactgctg agcaagtttg gggagcggat tccggccgag atggcgcgct
      601 tctacctggc ggagattgtc atggccatag actcggtgca ccggcttggc tacgtgcaca
      661 gggacatcaa acccgacaac atcctgctgg accgctgtgg ccacatccgc ctggccgact
      721 tcggctcttg cctcaagctg cgggcagatg gaacggtgcg gtcgctggtg gctgtgggca
      781 ccccagacta cctgtccccc gagatcctgc aggctgtggg cggtgggcct gggacaggca
      841 gctacgggcc cgagtgtgac tggtgggcgc tgggtgtatt
//
