LOCUS       sequence-51212-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Hussein R, Lim HN
  TITLE     Direct comparison of small RNA and transcription factor signaling.
  JOURNAL   Nucleic Acids Res. 2012 May 22.
  PUBMED    22618873
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2012 May 22."
COMMENT     Sequence Label: pHL 233
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     516..535
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    651..667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 catttaagtt gtttttctaa tccgcatatg atcaattcaa ggccgaataa gaaggctggc
       61 tctgcacctt ggtgatcaaa taattcgata gcttgtcgta ataatggcgg catactatca
      121 gtagtaggtg tttccctttc ttctttagcg acttgatgct cttgatcttc caatacgcaa
      181 cctaaagtaa aatgccccac agcgctgagt gcatataatg cattctctag tgaaaaacct
      241 tgttggcata aaaaggctaa ttgattttcg agagtttcat actgtttttc tgtaggccgt
      301 gtacctaaat gtacttttgc tccatcgcga tgacttagta aagcacatct aaaactttta
      361 gcgttattac gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa gtgagtatgg
      421 tgcctatcta acatctcaat ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc
      481 caatacaatg taggctgctc tacacctagc ttctgggcga gtttacgggt tgttaaacct
      541 tcgattccga cctcattaag cagctctaat gcgctgttaa tcactttact tttatctaat
      601 ctagacattt tttttcctcc ttagtgcgtc ctgctgatcc cgggagtatc ttgttatccg
      661 ctcacaatgt caatgttatc cgctcacatt tatggatcct gagcggatac atatttgaat
      721 gtatttagaa aaataaacaa aagagtttgt agaaacgcaa aaag
//
