LOCUS       sequence-51216-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Hussein R, Lim HN
  TITLE     Direct comparison of small RNA and transcription factor signaling.
  JOURNAL   Nucleic Acids Res. 2012 May 22.
  PUBMED    22618873
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2012 May 22."
COMMENT     Sequence Label: pHL 1571
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     521..540
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    637..653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tttcacattt aagttgtttt tctaatccgc atatgatcaa ttcaaggccg aataagaagg
       61 ctggctctgc accttggtga tcaaataatt cgatagcttg tcgtaataat ggcggcatac
      121 tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttga tcttccaata
      181 cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata taatgcattc tctagtgaaa
      241 aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt ttttctgtag
      301 gccgtgtacc taaatgtact tttgctccat cgcgatgact tagtaaagca catctaaaac
      361 ttttagcgtt attacgtaaa aaatcttgcc agctttcccc ttctaaaggg caaaagtgag
      421 tatggtgcct atctaacatc tcaatggcta aggcgtcgag caaagcccgc ttatttttta
      481 catgccaata caatgtaggc tgctctacac ctagcttctg ggcgagttta cgggttgtta
      541 aaccttcgat tccgacctca ttaagcagct ctaatgcgct gttaatcact ttacttttat
      601 ctaatctaga cattttcctc cttacccggg agtatcttgt tatccgctca caatgtcaat
      661 gttatccgct cacatttatg gatcctgagc ggatacatat ttgaatgtat ttagaaaaat
      721 aaacaaaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
//
