LOCUS       sequence-51219-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL601
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    253..269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(324..410)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    733..751
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    758..776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ttgtactacc ctgtacgatt aaagcttctt tcaggtacct tagtaactgg actgctggga
       61 tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt ccttcggggc
      121 atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg tgacctcatc
      181 cccaatcatg agggtctcac caactcgacg agtcagaatc agcatttttt ttcctcctta
      241 cccgggagta tcttgttatc cgctcacaat gtcaatgtta tccgctcaca tttatggatc
      301 cgcgcctagg tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
      361 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctctag
      421 cacgcgtacc atgggcccca gtatagattt gcggcggaat ctaacagaaa gcaagcaaag
      481 aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct taacgggtct
      541 tacaatcctt gcaggccgcc aacacaccct gtttttcctt tagccggaac aatccgtgtt
      601 gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat cctctgtctt
      661 cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg tcctcgctct
      721 atgtcgacag tatctctatc actgataggg atgtcaatct ctatcactga tagggactcg
      781 aggt
//
